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KMID : 0390219990100020224
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Journal of Clinical Otolaryngology, Head and Neck Surgery
1999 Volume.10 No. 2 p.224 ~ p.230
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Messenger RNA Expression of the Cytokine Gene Cluster in Human Fibroblast Activated with House Bust Mite Antigen
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유태현/Tai Hyun Yu
이봉희/최창용/주호범/옥미선/김광혁/Bong Hee Lee/Chang Yong Choi/Ho Bum Ju/Mee Sun Ock/Kwang Hyuk Kim
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Abstract
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Background and Objectives : It is well known that normal fibroblast can secrete a
group of cytokines, namely IL-lα, IL-1β, IL-6, IL-7, IL-8 and GM-CSF. The fibroblast
is a major component ol connective tissue in the airway and has many immunologic
roles. In case of allergic rhinitis, neutrophils, macrophages and lymphocytes are
infiltrated in nasal mucosa and these fells secrete several kinds of cytokines. These
reactions cause the activation of fibroblast growth abnormally and result in fibrosis.
Secreted cytokines also cause inflammatory reaction of connective tissue in nasal
mucosa. To identify the role of cytokines of identify in allergic rhinitis, we tired to find
the differences of cytokine secretion patterns and amounts between the normal and
allergic rhinitis patients fibroblast which was originated from inferior turbinate of nasal
cavity and primarily cultured in vitro.
Materials and Methods : Cultured human nasal fibroblast was activated with saline,
lipopolysaccharide(LPS, 5㎍/㎖) and house dust mite antigen (100㎍/㎖) for 4 hours.
Results : When normal fibroblast was treated with saline, mRNA expression of IL-lβ,
IL-6 and GM-CSF was confirmed. But the mRNA band of IL-6 was not found in the
patients group. Although it was identified that mRNA expression of IL-1β, IL-8 and
GM-CSF in both group, IL-8 mRNA expression was distinct in patient fibroblast
activated with LPS. When the normal fibroblast was activated with mite antigen, the
expression of IL-1β, IL-6, IL-8 and GM-CSF expression was identified. But IL-8
expression was suppressed in the patient fibroblast.
Conclusion : These results suggest that Il-1β, IL-6 and IL-8 could play as the
triggering factors inflammation and fibrosis in allergic rhinitis.
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KEYWORD
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